ࡱ > z bjbj . ucucz > > 0 $ 6 n M M M j M M M M (!u 3 M q 0 M Z = Z M Z M $ M M Z > B : Additional file 1. Analysis of the 39 CVS samples used for female vs male RNA-sequencing and DESeq2. (A) Principal components analysis shows male and female clusters. Principal components analysis plot of the 39 CVS samples used for RNA-seq shows clustering by cytogenetic sex. Blue: male samples, red: female samples. (B) Volcano plot of RNA-seq results before FPKM cutoff. DESeq2 results for sex differences in 39 CVS samples. Volcano plot for all genes detected by RNA-seq, without an FPKM cutoff for expression. Red points: genes significantly different between sexes (Benjamini-Hochberg procedure to control for false discovery rate, FDR<0.05). Yellow points: genes with 2-fold change or greater, |LogFC|>1 where FC = female/male fold-change. No genes fit yellow category. Blue points: genes which are both significantly different (FDR<0.05) and have a 2-fold change or greater difference in expression between males and females.Black points: other genes. (C) MA plot of RNA-seq results before FPKM cutoff. DESeq2 results for sex differences in 39 CVS samples, before an FPKM cutoff is applied. MA plot shows log2 of fold-changes (female/male) versus baseMean expression values (gene counts normalized by sequencing depth). Red points: genes significantly different between sexes (Benjamini-Hochberg procedure to control for false discovery rate, FDR<0.05). Black points: other genes. ` b f i j ? @ A B E F G { ͿͱveQ@@ h1 hW\ CJ OJ QJ ^J aJ &h1 hW\ 5CJ OJ QJ \^J aJ h hW\ CJ OJ QJ ^J aJ #h hW\ 5CJ OJ QJ ^J aJ hW\ 5CJ OJ QJ ^J aJ hW\ mH nH u j h6Z hW\ UmH nH u h2 CJ OJ QJ ^J aJ hW\ CJ OJ QJ ^J aJ h h CJ OJ QJ ^J aJ h 5CJ OJ QJ ^J aJ #h h 5CJ OJ QJ ^J aJ e @ B w x z $a$gdW\ # % v x y z İo h hW\ CJ OJ QJ ^J aJ jU2 h6Z hW\ UmH nH u hW\ CJ OJ QJ ^J aJ h1 hW\ CJ OJ QJ ^J aJ &h1 hW\ 5CJ OJ QJ \^J aJ hW\ 5CJ OJ QJ \^J aJ jű h6Z hW\ UmH nH u hW\ mH nH u hW\ hW\ CJ OJ QJ ^J aJ , 1h/ =!"#$% ű D d !! p s > A ? P i c t u r e 1 " b uB y k1r ݰ D nհ uB y k1rPNG IHDR sRGB pHYs .# .#x?v IDATx^g})[oN]rfSlKB %! ~IB%@Bxz .`$]ۦG^%흯}ڛOyO0x! IYM;˾" Q@ @ @ @ Xu$FW]a@ @ @ @ 11 N9; $F9@ @ @ @ @` ]u!g@ @ @ @ @( :.0 @ @ @ @ @ U'@btՅF @ @ @ @ HpJ9m@`Y.rV˲>º,Kc˽K>\xxyK LL[xm˷xJgk뼴U.5K*mfoٵZqL&*ZsuϘW.fT=l6 _ >4_5SQ