This is the data repository for the Homes_USA project (homes.yourwildlife.org).

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Scientific relevance
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Exploration of microorganisms, including potential allergens, associated with home dust indoors and outdoors.
- ~1,500 indoor and outdoor dust samples.
- Marker gene sequences (16S rRNA for bacteria, and ITS1 for fungi).

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Sampling background
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Dust samples were collected by volunteers participating in the Wild Life of Our Homes project (homes.yourwildlife.org), a citizen science project. Enrolled participants were provided a written informed consent form approved by the North Carolina State University’s Human Research Committee (approval no. 2177), as well as instructions for sampling and a sampling kit.

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How to access the raw data
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Data from this project are associated with NCBI BioProject PRJNA280363.

Raw sequencing data can be accessed via ftp (ftp://microbe.colorado.edu/Homes_USA/).

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File description
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- homes_mapping_file.txt --> Metadata associated with each sample including geographical coordinates, environmental variables and home description.

- otu_tables_wTax.zip --> Biom-format OTU tables for bacteria and fungi with taxonomic information. Sample ID appended with .I refers to indoor dust, while sample ID appended with .O refers to outdoor dust.

- rep_set_numbered.zip --> Fasta files containing representative OTU sequences for bacteria and fungi.

- indoor_indvalfilt_OTUnorm.zip --> Tab-delimited-format OTU tables for indoor-specific phylotypes. Indoor-specific phylotypes were selected using species indicator analyses (IndVal).

- fungi_patho.zip --> Proportion of fungal plant pathogens in outdoor dust. Distribution maps for the 20 most abundant species are included.
Interactive maps depicting the spatial distribution of the most abundant putative fungal plant pathogens in dust were generated using Tableau (www.tableau.com) and can be publicly accessed at https://public.tableau.com/profile/kristin.dietzel#!/vizhome/Top50FungalPlantPathogens/Top50Fungi