Annotated spectra of F_02_23_E2_MSA.msf

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Peptides

Confidence Sequence Activation Type Modifications IonScore ΔScore Rank Search Engine Charge Precursor m/z [Da] ΔM [ppm] First Scan Last Scan Annotated Spectrum Peak List
High sMSFSPDRVR CID S1(PhospR4) 29.24 0.07 1 Mascot (2) 2 631.27356 0.22 1022 1022 Image Peak List
High KITtQPINIR CID T4(PhospR4) 47.01 0.17 1 Mascot (2) 2 632.34570 1.60 949 949 Image Peak List
High FKVTSADLsPK CID S9(PhospR4) 23.34 1.00 1 Mascot (2) 2 636.81580 0.56 1040 1040 Image Peak List
High ENsGFGFLLTR CID S3(PhospR4) 38.47 1.00 1 Mascot (2) 2 660.80164 -1.86 3136 3136 Image Peak List
High DKDDFDDFcLR CID C9(Methylthio) 42.05 1.00 1 Mascot (2) 2 717.78729 0.56 2412 2412 Image Peak List
High RTSsIPDFEPVK CID S4(PhospR4) 34.23 1.00 1 Mascot (2) 2 728.34833 0.95 1325 1325 Image Peak List
High VDsEGVLcGANFK CID S3(PhospR4); C8(Methylthio) 61.91 1.00 1 Mascot (2) 2 732.79999 1.87 2492 2492 Image Peak List
High VPsPcLtPIGSGR CID S3(PhospR4); C5(Methylthio); T7(PhospR4) 38.31 0.55 1 Mascot (2) 2 745.30383 1.55 2354 2354 Image Peak List
High IELGLssDEDEK CID S6(PhospR4); S7(PhospR4) 30.40 1.00 1 Mascot (2) 2 747.78619 0.13 2034 2034 Image Peak List
High DKFGVPAVSLPsPK CID S12(PhospR4) 31.14 0.32 1 Mascot (2) 2 761.38849 -1.09 2088 2088 Image Peak List
High TRsFDDSPPAAAELR CID S3(PhospR4) 41.51 0.14 1 Mascot (2) 2 856.88947 4.44 1120 1120 Image Peak List
High sKSELEQcVTEESK CID S1(PhospR4); C8(Methylthio) 41.84 0.05 1 Mascot (2) 2 861.85272 1.11 1358 1358 Image Peak List
Medium TPsKISSIFcGTQDK CID S3(PhospR4); C10(Methylthio) 24.01 0.04 1 Mascot (2) 2 869.38324 0.23 2230 2230 Image Peak List
High KRGsFQNPSVMAVVR CID S4(PhospR4) 36.27 0.47 1 Mascot (2) 3 585.96362 2.18 1061 1061 Image Peak List
Medium FTNPsPcNsPVLQR CID S5(PhospR4); C7(Methylthio); S9(PhospR4) 19.33 1.00 1 Mascot (2) 2 883.34973 4.67 2104 2104 Image Peak List
High VHEGAPDTEVLLAsPR CID S14(PhospR4) 78.15 0.68 1 Mascot (2) 2 885.92554 0.85 1332 1332 Image Peak List
High VQTESGVMtAETGTYR CID T9(PhospR4) 41.96 0.24 1 Mascot (2) 2 905.39038 0.52 1164 1164 Image Peak List
High VQIESGVMtAETGTYR CID T9(PhospR4) 42.05 0.29 1 Mascot (2) 2 911.41187 4.13 1708 1708 Image Peak List
Medium RAPsPGGGSSTIVTLADR CID S4(PhospR4) 23.73 1.00 1 Mascot (2) 2 911.44861 2.40 1304 1304 Image Peak List
Medium TVAAVAGSPGTPtTPGsAR CID T13(PhospR4); S17(PhospR4) 17.27 0.06 1 Mascot (2) 2 929.41309 2.40 1045 1045 Image Peak List
High AQIsEPELAAEHEEVK CID S4(PhospR4) 49.70 1.00 1 Mascot (2) 2 930.42487 0.06 1129 1129 Image Peak List
High cAEKVPtPTNSYTGIR CID C1(Methylthio); T7(PhospR4) 26.04 0.08 1 Mascot (2) 2 931.91626 4.04 1220 1220 Image Peak List
High INsAEsMELWASQQK CID S3(PhospR4); S6(PhospR4) 39.60 1.00 1 Mascot (2) 2 941.37866 0.53 2564 2564 Image Peak List
High TKDsPVMGLLTEENTSK CID S4(PhospR4) 30.94 0.29 1 Mascot (2) 2 965.44586 -1.63 1752 1752 Image Peak List
Medium ALLEGGsDDEGASTEAQGR CID S7(PhospR4) 22.78 1.00 1 Mascot (2) 2 971.90717 2.25 1103 1103 Image Peak List
High SIPEGAVVcNVEHHVGDR CID C9(Methylthio) 34.68 1.00 1 Mascot (2) 3 655.30988 1.88 1428 1428 Image Peak List
High AHGTDPsPPMsPILGATR CID S7(PhospR4); S11(PhospR4) 53.13 0.32 1 Mascot (2) 2 982.92212 1.61 1834 1834 Image Peak List
High TPYHPMMDDDGsLsPR CID S12(PhospR4); S14(PhospR4) 26.00 1.00 1 Mascot (2) 2 989.86212 2.87 1484 1484 Image Peak List
Medium EDDKVALQEDNcADDVR CID C12(Methylthio) 24.00 1.00 1 Mascot (2) 3 660.94769 0.54 1150 1150 Image Peak List
Medium tNSSPGNFESPLDRPMR CID T1(PhospR4) 16.94 0.06 1 Mascot (2) 2 992.93323 0.41 1498 1498 Image Peak List
High NMQQLNQsPDIDGELSK CID S8(PhospR4) 47.30 1.00 1 Mascot (2) 2 998.94067 2.91 1675 1675 Image Peak List
High GRsPAFNALAATFESQNAR CID S3(PhospR4) 80.62 0.79 1 Mascot (2) 2 1044.49036 3.59 2307 2307 Image Peak List
High LIHPLPPALSsPETsPER CID S11(PhospR4); S15(PhospR4) 29.93 0.23 1 Mascot (2) 3 700.99768 0.83 2116 2116 Image Peak List
High AFAHPPEELNsPASHFSGK CID S11(PhospR4) 32.61 0.39 1 Mascot (2) 3 701.64893 -0.48 1206 1206 Image Peak List
High VGSSsGKPTKPVVVADcGQLS CID S5(PhospR4); C17(Methylthio) 36.53 0.03 1 Mascot (2) 2 1071.50562 3.17 1719 1719 Image Peak List
High AVGsHcsLSEMDDLDLTR CID S4(PhospR4); C6(Methylthio); S7(PhospR4) 42.11 0.12 1 Mascot (2) 2 1077.90198 1.06 3021 3021 Image Peak List
High LRSDtMIKDSSNESQEIK CID T5(PhospR4) 27.00 0.04 1 Mascot (2) 2 1080.99475 -0.98 820 820 Image Peak List
High GRLELSAVPSSYSsGQLDPK CID S14(PhospR4) 25.36 0.08 1 Mascot (2) 2 1086.02490 2.04 1782 1782 Image Peak List
High HMIQEGSSMFYHQPSsVK CID S16(PhospR4) 44.66 1.00 1 Mascot (2) 3 724.98224 3.61 1152 1152 Image Peak List
Medium TSsFRDPLSVSDSASPIPSR CID S3(PhospR4) 18.23 0.06 1 Mascot (2) 3 729.34161 -1.06 1988 1988 Image Peak List
High VLSHAVSEsDVSVHSTFASR CID S9(PhospR4) 61.87 0.10 1 Mascot (2) 2 1098.01135 1.14 1173 1173 Image Peak List
Medium STTTTVsSVHSPTTDQDFSK CID S7(PhospR4) 18.60 1.00 1 Mascot (2) 3 735.98914 -0.88 788 788 Image Peak List
High FSMDAGAFDAVVcSHHAHSGK CID C13(Methylthio) 37.63 1.00 1 Mascot (2) 3 740.65332 1.52 1815 1815 Image Peak List
High KPEDQNISGFMSAGsPVLNR CID S15(PhospR4) 28.47 0.36 1 Mascot (2) 3 743.01270 0.95 1946 1946 Image Peak List
High FLVGVKPADDFLDsHSSPPK CID S14(PhospR4) 35.70 0.06 1 Mascot (2) 3 746.02802 0.36 2097 2097 Image Peak List
Medium LVPHTVASQSEVDVAsPVSEK CID S16(PhospR4) 15.87 1.00 1 Mascot (2) 3 753.70221 0.68 1157 1157 Image Peak List
High SAsFKEESDFFADLKESEK CID S3(PhospR4) 30.62 0.03 1 Mascot (2) 3 758.66724 0.92 2296 2296 Image Peak List
High VLSHAVSEsDVsVHSTFASR CID S9(PhospR4); S12(PhospR4) 28.79 0.17 1 Mascot (2) 3 758.99640 -2.03 1369 1369 Image Peak List
High SsDSLSGTNELLNINSETPMK CID S2(PhospR4) 55.26 0.02 1 Mascot (2) 2 1159.01770 0.65 2671 2671 Image Peak List
High LSEDTGsPHHHADILMVQEK CID S7(PhospR4) 33.71 0.29 1 Mascot (2) 3 775.35046 -0.41 783 783 Image Peak List
Medium WDPDsDMDVDDDRKPIPSR CID S5(PhospR4) 22.53 1.00 1 Mascot (2) 3 780.32806 2.11 1365 1365 Image Peak List
High DFVVAHNLsGALQSDGRPVAPR CID S9(PhospR4) 36.61 0.46 1 Mascot (2) 3 796.06085 0.65 1948 1948 Image Peak List
High TGIWEYGDIQsDDEDNVPVR CID S11(PhospR4) 33.50 0.47 1 Mascot (2) 3 796.67273 0.33 2685 2685 Image Peak List
High KLALcGGEGGQEWDDDVYEGVR CID C5(Methylthio) 29.37 1.00 1 Mascot (2) 3 814.69623 1.63 2370 2370 Image Peak List
Medium KQEEAPsTPQRPLSPLGEAcSR CID S7(PhospR4); C20(Methylthio) 21.52 0.05 1 Mascot (2) 3 836.38562 0.14 1381 1381 Image Peak List
High RQGSGGVWGWLAGSPQEKDDDsP CID S22(PhospR4) 50.13 1.00 1 Mascot (2) 3 837.03320 2.77 2706 2706 Image Peak List
High SFDRESPsNLDSTSGISQPHNR CID S8(PhospR4) 44.52 0.09 1 Mascot (2) 3 837.70074 -0.80 792 792 Image Peak List
High FGFDDAFNYKEEsDLTAALKR CID S13(PhospR4) 32.05 0.44 1 Mascot (2) 3 839.71429 -2.04 2879 2879 Image Peak List
Medium TKNsNHDENEFISFEPNQNTK CID S4(PhospR4) 25.53 0.04 1 Mascot (2) 3 858.36932 -0.27 1309 1309 Image Peak List
High AHEGEDEGAGLsELEEEDDDAKR CID S12(PhospR4) 47.34 1.00 1 Mascot (2) 3 861.01331 1.65 1194 1194 Image Peak List
High SDVAsVASADEFEYKDPVDGSISK CID S5(PhospR4) 30.90 0.39 1 Mascot (2) 3 866.05206 2.97 2102 2102 Image Peak List
Medium ADVQEtLLKPSKPSQSKADVQEK CID T6(PhospR4) 16.40 1.00 1 Mascot (2) 3 869.44452 4.44 2125 2125 Image Peak List
Medium VTYVAPPRPPsPVHEGSEEGSSPR CID S11(PhospR4) 21.51 1.00 1 Mascot (2) 3 871.40881 -0.88 938 938 Image Peak List
High VASSGILPSSGSGSDRQSPLHDSTsK CID S25(PhospR4) 29.95 0.10 1 Mascot (2) 3 879.74097 -2.64 736 736 Image Peak List
High TPEINKAPEVNAPTPVSLsPKPDR CID S19(PhospR4) 26.04 0.08 1 Mascot (2) 3 879.78333 2.62 1367 1367 Image Peak List
Medium ELLSELKSEEGDGtPHSSASPFSR CID T14(PhospR4) 15.91 1.00 1 Mascot (2) 3 880.73120 -1.35 1759 1759 Image Peak List
Medium LADTsDGEMDGDPPYSHDHPMPQG CID S5(PhospR4) 21.85 0.23 1 Mascot (2) 3 883.67010 1.93 1271 1271 Image Peak List
High TLPVAVVEVVKPEsPVLVIVEKPK CID S14(PhospR4) 36.97 1.00 1 Mascot (2) 3 883.84357 -3.10 2788 2788 Image Peak List
High SPTVVTVQPSsPRFPISTPTAGAQR CID S11(PhospR4) 50.48 0.12 1 Mascot (2) 3 887.78461 -0.17 1934 1934 Image Peak List
High VHDMVVADADQDDGtDDDNDLGEK CID T15(PhospR4) 33.50 1.00 1 Mascot (2) 3 890.34656 2.14 1213 1213 Image Peak List
High HPQWQSDDGGDNsEPEsPSDSLR CID S13(PhospR4); S17(PhospR4) 30.42 0.30 1 Mascot (2) 2 1350.50085 2.23 1208 1208 Image Peak List
High KRsAPTTPINQNAAAAFAAVSEEER CID S3(PhospR4) 106.27 0.15 1 Mascot (2) 3 903.77374 2.71 1990 1990 Image Peak List
Medium LQTFSsFQKPLEPPNNNAPPRPR CID S6(PhospR4) 18.29 1.00 1 Mascot (2) 3 905.78558 0.66 1523 1523 Image Peak List
High RANsYHSYSQSPPYDYQYEER CID S4(PhospR4) 34.98 0.17 1 Mascot (2) 3 907.37146 -1.88 954 954 Image Peak List
High VEEEKPAVPAAEEEKSsEAAPVETK CID S17(PhospR4) 35.80 1.00 1 Mascot (2) 3 912.09589 0.85 575 575 Image Peak List
High IStPELPPPVFIKPEsPEPVSTPK CID T3(PhospR4); S16(PhospR4) 33.40 1.00 1 Mascot (2) 3 916.11578 -2.38 2921 2921 Image Peak List
High FPDDKDGEDEKSGDsGGENLMDSDK CID S15(PhospR4) 43.62 0.27 1 Mascot (2) 3 923.02429 0.77 896 896 Image Peak List
Medium EHNAVcDALKDEDDDLEDEDLYR CID C6(Methylthio) 20.52 1.00 1 Mascot (2) 3 923.38275 1.37 2466 2466 Image Peak List
High ADsVEGTVVDRDGDEVNDDSAGPSKR CID S3(PhospR4) 28.98 0.21 1 Mascot (2) 3 924.06720 2.51 706 706 Image Peak List
Medium SHLRPPGNISGSQsPPVESPGSYHSK CID S14(PhospR4) 16.54 1.00 1 Mascot (2) 3 928.10370 0.72 638 638 Image Peak List
High VEEKKEEsDEEDYEGGFGLFDEE CID S8(PhospR4) 37.31 0.38 1 Mascot (2) 3 930.36804 0.76 2571 2571 Image Peak List
High AHGTAVGLPSEDDMGNsEVGHNALGAGR CID S17(PhospR4) 59.20 0.41 1 Mascot (2) 3 933.74396 0.70 1334 1334 Image Peak List
High RANsYHSYSQSPPyDYQYEER CID S4(PhospR4); Y14(Phospho) 37.92 0.21 1 Mascot (2) 3 934.02942 0.87 1187 1187 Image Peak List
High ITSNNMDHHScAEEYsDEDVEVR CID C11(Methylthio); S16(PhospR4) 26.01 1.00 1 Mascot (2) 3 936.01965 0.59 1054 1054 Image Peak List
High TGIWEYGDIQsDDEDNVPVRKPGR CID S11(PhospR4) 31.12 1.00 1 Mascot (2) 3 942.76569 3.30 1850 1850 Image Peak List
High KPPPPPPHTSSSSSsPVFDDASPQQSK CID S15(PhospR4) 25.66 0.23 1 Mascot (2) 3 957.10767 1.51 475 475 Image Peak List
High HLESFNFDSEIPADLPVTsSSHELK CID S19(PhospR4) 46.43 0.13 1 Mascot (2) 3 960.44434 1.68 2517 2517 Image Peak List
High DSYQNDYQEEDDSSGSGTVVIRsPR CID S23(PhospR4) 39.35 0.36 1 Mascot (2) 3 962.07092 2.64 1171 1171 Image Peak List
Medium FEDQSETSSsHGGItAPGPISVAQLPR CID S10(PhospR4); T15(PhospR4) 23.37 0.04 1 Mascot (2) 3 976.76978 4.58 2573 2573 Image Peak List
Medium VSsTLSEAHGDAAHcFTLYEIEEATK CID S3(PhospR4); C15(Methylthio) 15.75 1.00 1 Mascot (2) 3 979.08972 0.66 2783 2783 Image Peak List
High LAPAEAVVEDNQKAsDIEEGDEVDSKK CID S15(PhospR4) 46.98 0.60 1 Mascot (2) 3 989.45135 -4.07 1192 1192 Image Peak List
High SLsENDAGRNELFESPVEVEDHNSSK CID S3(PhospR4) 43.80 0.39 1 Mascot (2) 3 990.42896 -2.51 1698 1698 Image Peak List
High LSKSDNSLSsQFVTSESPANTAQQDSGK CID S10(PhospR4) 40.73 0.05 1 Mascot (2) 3 998.45490 4.30 1444 1444 Image Peak List
High ISHSTGSETLDQNMVLGEVsPKDEESR CID S20(PhospR4) 52.06 0.37 1 Mascot (2) 3 1009.12219 2.07 1477 1477 Image Peak List
High IELQSQSWPMQQsFSPEKENGQTGAR CID S13(PhospR4) 38.74 0.05 1 Mascot (2) 3 1015.12885 3.39 2142 2142 Image Peak List
High KLEVVsPVPADIDIANSVEPLHISEIAK CID S6(PhospR4) 73.07 0.44 1 Mascot (2) 3 1021.87482 2.74 2926 2926 Image Peak List
High KVQEQPHVEQESAFSTRPSPARPVsAK CID S25(PhospR4) 27.52 0.04 1 Mascot (2) 3 1024.17651 1.39 430 430 Image Peak List
High VHDDDEDVssEDENETHNSNAVYYK CID S9(PhospR4); S10(PhospR4) 57.74 0.29 1 Mascot (2) 3 1024.37598 1.09 720 720 Image Peak List
High IEEEsENEEEEEGEEEDDDEEVKEK CID S5(PhospR4) 53.30 1.00 1 Mascot (2) 3 1036.05518 2.61 652 652 Image Peak List
High GMVSSGGPVsPGPVYPGGRPGAGGLMPGMPGTR CID S10(PhospR4) 41.44 0.44 1 Mascot (2) 3 1039.81897 -1.49 2310 2310 Image Peak List
High SAANDTEMDDDsGNEKAGDDLSQDEIGVR CID S12(PhospR4) 66.41 0.17 1 Mascot (2) 3 1045.41785 0.39 1393 1393 Image Peak List
High FHSMEKPQFDHQNsSGSNYDDLTPQR CID S14(PhospR4) 31.14 0.10 1 Mascot (2) 3 1049.11023 1.74 1019 1019 Image Peak List
Medium HPSIVVTPADSDPLFAPPSYYSESRsPR CID S26(PhospR4) 21.42 1.00 1 Mascot (2) 3 1051.49390 -1.73 2312 2312 Image Peak List
High HDAIASGSPLPVQASGSPLSVQASKPADsSPK CID S29(PhospR4) 33.39 0.03 1 Mascot (2) 3 1056.18945 3.80 1598 1598 Image Peak List
High STSsNPADDIPLTEAKDEEEASEVAGLEAK CID S4(PhospR4) 38.59 0.46 1 Mascot (2) 3 1061.80957 1.24 2510 2510 Image Peak List
High WSPtKPSPQSANQSMNYSVAQSGQSQTSR CID T4(PhospR4) 50.85 0.10 1 Mascot (2) 3 1073.81116 0.84 1241 1241 Image Peak List
Medium EKPITDKEVTIPtPVAEKEEVAAPVSDEK CID T13(PhospR4) 16.84 1.00 1 Mascot (2) 3 1077.20911 -0.04 1404 1404 Image Peak List
High RKGDGDDEEsEDESAENVVNVEDFLVQK CID S10(PhospR4) 63.91 0.36 1 Mascot (2) 3 1078.13330 1.56 2625 2625 Image Peak List
High TRDsDDDEVHDRDYDLAGLANNLNQFR CID S4(PhospR4) 35.93 0.25 1 Mascot (2) 3 1082.14038 4.23 2396 2396 Image Peak List
High RASLsTGNIPEMFDGRPSFTEGSGLEASPR CID S5(PhospR4) 32.55 0.12 1 Mascot (2) 3 1082.83667 2.08 2445 2445 Image Peak List
High STssNPADDIPLTEAKDEEEASEVAGLEAK CID S3(PhospR4); S4(PhospR4) 55.85 0.02 1 Mascot (2) 3 1088.46204 -1.53 2916 2916 Image Peak List
High GMGTNLFDKPSHPNsPTVYDWLYSDDTR CID S15(PhospR4) 57.50 0.02 1 Mascot (2) 3 1098.48267 2.67 2856 2856 Image Peak List
High NGQIGIAHNPLWYEPYDPSDPDDVEGcNR CID C27(Methylthio) 39.16 1.00 1 Mascot (2) 3 1106.48450 3.78 2611 2611 Image Peak List
High TASNDESSSFDGHDEEDsVEHDESTADTAR CID S18(PhospR4) 54.01 0.33 1 Mascot (2) 3 1107.41785 4.02 797 797 Image Peak List
Medium KTDGSTTPAyAHGQHHSIFSPATGAVSDSSLK CID Y10(Phospho) 22.86 0.17 1 Mascot (2) 3 1112.51245 -3.04 994 994 Image Peak List
High HDSSSSYKsDADDDVDFSETTSNPTWLEK CID S9(PhospR4) 69.95 0.20 1 Mascot (2) 3 1115.12231 2.65 1967 1967 Image Peak List
High YQSTYEGYGsPIREEPPPPYSYSEPQSR CID S10(PhospR4) 49.64 0.30 1 Mascot (2) 3 1115.48279 -2.46 1820 1820 Image Peak List
High STPAKNsDSEEMFDsDGEDEEEDKEVAVK CID S7(PhospR4); S15(PhospR4) 42.69 0.26 1 Mascot (2) 3 1126.43298 0.91 1257 1257 Image Peak List
Medium TASNDESSsFDGHDEEDsVEHDESTADTAR CID S9(PhospR4); S18(PhospR4) 19.16 1.00 1 Mascot (2) 3 1134.07068 1.62 900 900 Image Peak List
High YGGDAGDRsPTHYPQAEEGEDEDEVNNLFK CID S9(PhospR4) 63.61 0.14 1 Mascot (2) 3 1140.47583 4.56 1878 1878 Image Peak List
Medium EISHDssDEEDGDGGKNTNEMLNYGLTIASK CID S6(PhospR4); S7(PhospR4) 23.90 0.17 1 Mascot (2) 3 1162.80798 4.73 2541 2541 Image Peak List
High DAPAEsDAEEEAEDDDNEGDDSDNESKSEETK CID S6(PhospR4) 104.27 0.67 1 Mascot (2) 3 1184.76123 3.28 669 669 Image Peak List
High STssSPPPPPPSSSLPQQEQEQDQQQLPLRR CID S3(PhospR4); S4(PhospR4) 53.44 0.11 1 Mascot (2) 3 1196.21191 -0.01 1563 1563 Image Peak List
High SNASVGNGSVEDANTDASEVSKDDAGPAsEEEKSEA CID S29(PhospR4) 61.59 0.08 1 Mascot (2) 3 1211.83301 0.31 1003 1003 Image Peak List
High YEEMNKPSAPLTSHEPAMIPVAEEPDDsPIHGR CID S28(PhospR4) 48.18 1.00 1 Mascot (2) 3 1242.22937 2.01 1666 1666 Image Peak List
Medium ELDSSSEAVSGNSDKVGADDLsDSEKEKPNLVGDGK CID S22(PhospR4) 25.18 0.08 1 Mascot (2) 3 1253.56580 1.66 1127 1127 Image Peak List
High SSAVQEEQFGEHTEPcSSEIKDESHGKEESVEVK CID C16(Methylthio) 38.04 1.00 1 Mascot (2) 3 1274.56726 3.09 1101 1101 Image Peak List
High IDLDKPEVEDDDDNDEDDsEDDDEAEGHDGEAGGR CID S19(PhospR4) 77.74 1.00 1 Mascot (2) 3 1304.81018 0.99 1134 1134 Image Peak List
High DAETVNQTSHPTEEEAQVTVSSNADVEDSHETVsPR CID S34(PhospR4) 84.46 0.02 1 Mascot (2) 3 1325.90930 3.33 1148 1148 Image Peak List
High KDNADMTSDLTGTIESADSAIPNsPTEDAAPGNIQDLK CID S24(PhospR4) 73.39 0.14 1 Mascot (2) 3 1328.26575 1.95 2760 2760 Image Peak List
High AEDEEDAsDFEPEENGVEEDIDEGEDDENDNSGGAGK CID S8(PhospR4) 86.19 1.00 1 Mascot (2) 3 1346.49390 4.60 2333 2333 Image Peak List
High FAAPSSLsEDDDDDDDDDPDYVEEEEEPLVSHRPR CID S8(PhospR4) 83.60 0.10 1 Mascot (2) 3 1362.54407 -0.72 2111 2111 Image Peak List
High GMDVDEDDsDDDEEEDsEDEEEEETPKKPEPINK CID S9(PhospR4); S17(PhospR4) 67.16 0.40 1 Mascot (2) 3 1366.82800 -1.11 980 980 Image Peak List

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Search Summary

Workflow created with Discoverer version: 1.2.0.208 (DBVersion:53)

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Search name: F15_CS23_incl_022012_Excl2_MSA
Search description: -
Search date: 03/25/2013 10:14:15

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The pipeline tree:
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    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Mascot

================================================================================

Search name: F15_CS23_incl_022012_Excl2_MSA
Search description: -
Search date: 03/25/2013 10:14:15

================================================================================

The pipeline tree:
------------------

    |-(0) Spectrum Files
        |-(1) Spectrum Selector
            |-(2) Mascot

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Processing node 0: Spectrum Files
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Input Data:
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File Name(s): F:\Raw files\022012\F15_CS23_incl_022012_Excl2.raw

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Processing node 1: Spectrum Selector
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1. General Settings:
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Precursor Selection: Use MS1 Precursor
Use New Precursor Reevaluation: True

2. Spectrum Properties Filter:
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Lower RT Limit: 0
Upper RT Limit: 0
Lowest Charge State: 0
Highest Charge State: 0
Min. Precursor Mass: 400 Da
Max. Precursor Mass: 10000 Da
Total Intensity Threshold: 0
Minimum Peak Count: 1

3. Scan Event Filters:
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MS Order: Is MS2
Activation Type: Is CID
Scan Type: Is Full

4. Peak Filters:
-----------------------------
S/N Threshold (FT-only): 3

5. Replacements for Unrecognized Properties:
-----------------------------
Unrecognized Charge Replacements: Automatic
Unrecognized Mass Analyzer Replacements: ITMS
Unrecognized MS Order Replacements: MS2
Unrecognized Activation Type Replacements: CID
Unrecognized Polarity Replacements: +

------------------------------------------------------------------------------
Processing node 2: Mascot
------------------------------------------------------------------------------

1. Input Data:
-----------------------------
Protein Database: TAIR10
Enzyme Name: Trypsin
Maximum Missed Cleavage Sites: 2
Instrument: ESI-TRAP
Taxonomy: All entries

1.1 Peptide Scoring Options:
-----------------------------
Peptide Cut Off Score: 10

1.2 Protein Scoring Options:
-----------------------------
Use MudPIT Scoring: True
Protein Relevance Threshold: 20
Protein Relevance Factor: 1

2. Decoy Database Search:
-----------------------------
Decoy Search: True

3. Tolerances:
-----------------------------
Precursor Mass Tolerance: 5 ppm
Fragment Mass Tolerance: 0.6 Da
Use Average Precursor Mass: False

4. Decoy Database Search:
-----------------------------
Significance High: 0.04
Significance Middle: 0.609

4. Dynamic Modifications:
-----------------------------
1. Dynamic Modification: Phosphogood (ST)
2. Dynamic Modification: Phospho (Y)

5. Static Modifications:
-----------------------------
1. Static Modification: Methylthio (C)

================================================================================

Processing details:

03/25/2013 10:23 AM (2):Mascot: Search completed
03/25/2013 10:23 AM (2):Mascot: 249 protein(s) scored and inserted into result file in 5.86 s
03/25/2013 10:23 AM (2):Mascot: ScoreCalculator: 249 protein(s) scored
03/25/2013 10:23 AM (2):Mascot: Result finalization started
03/25/2013 10:23 AM (2):Mascot: Start calculating relaxed FDR
03/25/2013 10:23 AM (2):Mascot: Start calculating strict FDR
03/25/2013 10:23 AM (2):Mascot: Used mascot server http://mascot/mascot/ with Mascot version 2.2.7
03/25/2013 10:23 AM (2):Mascot: Sending 278 peptide hits (686 peptides) to result file
03/25/2013 10:23 AM (2):Mascot: Sending 28 decoy peptide hits (43 peptides) to result file
03/25/2013 10:23 AM (2):Mascot: Reading decoy results
03/25/2013 10:23 AM (2):Mascot: Start translating results
03/25/2013 10:23 AM (2):Mascot: Start mapping modifications
03/25/2013 10:22 AM (2):Mascot: Received 286 proteins from Mascot server
03/25/2013 10:22 AM (2):Mascot: Start mapping 286 proteins
03/25/2013 10:22 AM (2):Mascot: Start parsing results
03/25/2013 10:22 AM (2):Mascot: Received Mascot result file (filename=../data/20130325/F007456.dat)
03/25/2013 10:22 AM (2):Mascot: Mascot Server completed
03/25/2013 10:20 AM (2):Mascot: Mascot result on server (filename=../data/20130325/F007456.dat)
03/25/2013 10:20 AM (2):Mascot: Start searching 3865 spectra
03/25/2013 10:20 AM (2):Mascot: Receiving spectra 3001 to 3866.
03/25/2013 10:20 AM (1):Spectrum Selector: Sending 865 spectra to Spectrum Distributor
03/25/2013 10:20 AM (1):Spectrum Selector: Sending 865 spectra to result file
03/25/2013 10:20 AM (2):Mascot: Receiving spectra 2001 to 3001.
03/25/2013 10:20 AM (1):Spectrum Selector: Sending 1000 spectra to Spectrum Distributor
03/25/2013 10:20 AM (1):Spectrum Selector: Sending 1000 spectra to result file
03/25/2013 10:20 AM (2):Mascot: Receiving spectra 1001 to 2001.
03/25/2013 10:20 AM (1):Spectrum Selector: Sending 1000 spectra to Spectrum Distributor
03/25/2013 10:19 AM (1):Spectrum Selector: Sending 1000 spectra to result file
03/25/2013 10:19 AM (2):Mascot: Use mascot server http://mascot/mascot/ with Mascot version 2.2.7
03/25/2013 10:19 AM (2):Mascot: Receiving spectra 1 to 1001.
03/25/2013 10:19 AM (1):Spectrum Selector: Sending 1000 spectra to Spectrum Distributor
03/25/2013 10:19 AM (1):Spectrum Selector: Sending 1000 spectra to result file


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