FILE INVENTORY Dataset: Ruiz, Kaitlyn; Bruce, Alexander; Chérémond, Nervah C.; Stratton, Chase A.; Murrell, Ebony G.; Gillette, Samantha; Morrison, William R. (2022). Data from: Field Trapping and Flight Capacity of Eucosma giganteana (Riley) (Lepidoptera: Tortricidae) in Response to Behaviorally Active Congeneric Semiochemicals in Novel Silflower Agroecosystems. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1526070. ++++++ File Title: Field Baited Sticky Card Assay File Name: eucosma_sticky_card_captures_raw_data_2019_2020.csv File Description: Eight semiochemicals were identified as attractants from other related Eucosma spp. through the primary literature [13–16,20–24] and searching Pherobase. Semiochemi-cals were purchased from ALFA Chemistry (Ronkonkoma, NY, USA), TRC Chemistry (North York, Ontario, Canada), and Bedoukian Inc. (Danbury, CT, USA), and formulated in behaviorally-relevant concentrations emitted by other Eucosma spp. Each of the eight semiochemicals was serially diluted with acetone and 2.5 mL of each was pipet-ted in 3-mL LDPE dropping bottles (Wheaton, DWK Life Sciences, LLC, Millville, NJ, USA). Concentrations varied from 0.004 to 0.02 µg/μL, depending on the behaviorally rel-evant dose (see above). Each was compared to a negative control consisting of 2.5 mL of acetone (solvent only). Each field had three transects spaced at least 10 m apart, each with a full set of semi-ochemical treatments represented. Each trap within the transect was spaced 10 m apart. Each trap consisted of a 1.27-cm diameter PVC pipe hammered in row with the silflower to a finished height of 1 m, in line with the canopy of S. integrifolium. A single 30.4 cm × 30.4 cm clear sticky card (Alpha Scents, Canby, OR, USA) was folded in half and inserted in a 271 cm long sticky card ring holder (Olson Products Inc., Medina, OH, USA). The ring holder was bent at a 90° angle to wedge the card holder upright in position, which was subsequently wedged in the opening at the top of the PVC pipe. To protect against dis-lodgement by wind, sticky cards were affixed to the top of the metal card holder with a 50 mm × 30 mm binder clip. A single, capped LDPE 3-mL dropping bottle with one of the semiochemical treatments above was inserted in the top of the PCV pipe opening and af-fixed in place by tying it to the card holder with garden wire. Every week, the lures were replaced with a freshly prepared treatment and the position of the lure was rotated in the transect every two weeks. Traps were rotated because of the short duration of the flying season and resulted in every treatment occupying every position at least once. Sticky cards were changed on a weekly basis after the first recorded capture of an E. giganteana adult. Traps were deployed 7 June 2019 to 14 August 2019 and 15 June 2020 to 10 August 2020. In total, there were n = 3 replicates of each semiochemical treatment per field site. The number of E. giganteana and Lepidopteran nontargets was counted on each sticky card af-ter freezing cards at −20 °C for at least 24 h. ++++++ File Title: Flight Mill Assay - 24 h period File Name: flight_mill_data_full_day_eucosma.csv File Description: Six flight mills were situated on a 71.1 cm × 91.4 cm sheet of nonporous plastic to minimize vibrations. Each mill was spaced 39 cm apart in rows (e.g., 3 in a row), and 25.4 cm between rows. Each mill contained three wires connected to the main connector board (#777101-01, National Instruments, Austin, TX, USA). A DC Power Supply (QW-MS3010D, Wuxi Qiaowei Eelectroeics Co Ltd., Wuxi, Jiangsu, China) was set to the site and connected to the main wiring block to provide power. A 50-pin ribbon cable (180524-10, National Instruments) ran from the main wiring connector block into a spe-cially made PC port (77690-01, National Instruments), where a computer with the soft-ware Labview 2017 (version 17.0.1f3, National Instruments) automatically recorded the data from the mills. Each mill consisted of a PTFE cylinder with a head suspended by two antipodal magnets to create levitation and connected by an insect pin with lubricant to ensure frictionless turning. At the top of the head, 2 14 cm hollow tubes (total flight diam-eter was 28 cm and circumference was 0.86 m) extended perpendicularly with right an-gles formed at the end for insect attachment. Protruding from the bottom of the head was a magnet to activate the Halls Sensor placed on the base of the mill during rotation. Levels on the bottom of the mills and adjustable feet ensured that mills were even relative to cen-ter of gravity. Lighting was uniformly provided overhead using full spectrum lights. Six adults were run simultaneously on the six flight mills described above (15-FMASM SDP Unit, Crist Instrument Co., Hagerstown, MD, USA) to test flight capacity. A 14-gauge copper wire was stripped into individual threads and cut into 4 cm segments. The copper wire thread was wrapped around insect pin (#6 insect pin, BioQuip Products, Rancho Dominguez, CA, USA) embedded in modelling clay to form a loop. The loop was pinched with a standard metal forceps, then excess wire was ablated on the shorter end with scissors, and the loop was flattened relative to the plane of gravity. Moths were placed singly on a metal mason jar lid on top of ice and restrained with a plastic lid for a 59.1-mL container until sessile. Afterwards, the pronotum of E. giganteana was descaled lightly with an artist’s paintbrush; then the copper loop was dipped in instant adhesive (#347908, Evo Stik Multi-Purpose Impact Adhesive, Bostik, Ltd., Leicester, UK) and affixed onto the pronotum of the adult, ensuring not to impair proper wing functioning, and avoiding the eyes and antennae of the adults. The individual was tethered by inserting the point of the copper wire of the hypodermic needle attached to the rotation arm of each flight mill. Each trial was started between 15:00 and 18:00 by gently blowing on the insect to initiate flight and run for 24 h in parallel. Assays were conducted at 21.4 ± 0.01 °C tem-perature and 54.2 ± 0.2% RH and monitored with a datalogger (UX100-011, Hobo, temp/RH logger, Onset, Bourne, MA, USA). The semiochemical treatments in the flight mill assay included (E)-8-dodecenyl acetate, (Z)-9-dodecenyl acetate, and an unbaited control (acetone solvent only). Semiochemicals were freshly prepared in LDPE dropping bottles, as in the field-baiting experiment (as above), and placed in the center among the flight mills on a nonporous glass surface to prevent contamination. A smoke test con-firmed that volatiles came into contact with each flight mill. Between runs, surfaces on the flight mill were thoroughly wiped down with solvent and allowed to dry and dissipate before use again. There were 12–18 replicates per treatment. At the end of a trial, insects were detached and weighed on a balance. Data were streamed in real time to a computer which automatically record the flight parameters: distance flown, the number of tandem flight bouts over the sampling interval (flights lasting more than 1 s), mean flight bout duration, and mean distance flown per bout. Data were also parsed by time of date to de-termine maximum time of dispersal. Data were analyzed with R software. ++++++ File Title: Flight Mill Assay - Diurnal Time Course File Name: flight_mill_data_period_diurnal_eucosma.csv File Description: Broken down by optimal flight period (morning, afternoon, or night), but otherwise equivalent to 24 h period. . Periods are defined as follows: Morning: 4:00–11:59; Afternoon: 12:00–19:59; Night: 20:00–3:59.