Ecological Archives E088-032-A7

A. Elizabeth Arnold and F. Lutzoni. 2007. Diversity and host range of foliar fungal endophytes: Are tropical leaves biodiversity hotspots? Ecology 88:541–549.

Appendix G. Instability in BLAST-based identifications at the genus and family levels for foliar endophytes from two focal tropical hosts (Heisteria concinna, Ouratea lucens).

Primary BLAST analyses were performed in January 2001. The same sequences were subjected to BLAST analysis in August 2005. Changes in genus- and family-level identifications are common, reflecting the growth and refinement of the GenBank database. Recent BLAST results also recover a large proportion of unidentified taxa, reflecting the inclusion of a growing number of environmental samples and unidentified fungi in GenBank. Placement based on 2005 BLAST analyses was confirmed for isolates using phylogenetic methods (Arnold et al. 2007, Higgins et al. 2007).

Host

Genus (2001)

Family (2001)

Genus (2005)

Family (2005)

Top match (2005) with match score and e-value

 

Heisteria

Arthrinium

Apiosporaceae

Pestalotiopsis

Amphisphaeriaceae

gb|AF409991.1|  Pestalotiopsis sp. 673    0.0 

Heisteria

Arthrinium

Apiosporaceae

Pestalotiopsis

Amphisphaeriaceae

gb|AY681481.1|  Pestalotiopsis theae 687    0.0 

Ouratea

Arthrinium

Apiosporaceae

Xylaria

Xylariaceae

gb|AF153740.1|AF153740  Xylaria sp. MS1033 887    0.0 

Heisteria

Arthrinium

Apiosporaceae

Hypoxylon

Xylariaceae

emb|AJ390409.1|HAN390409  Hypoxylon stygium 1096    0.0 

Ouratea

Botryosphaeria

Botryosphaeriaceae

Guignardia

Botryosphaeriaceae

gb|AY601899.1|  Fungal endophyte sp. 1185    0.0 

Ouratea

Botryosphaeria

Botryosphaeriaceae

Phyllosticta

Botryosphaeriaceae

gb|AF532314.1|  Phyllosticta sp. MSR-1 850    0.0 

Ouratea

Botryosphaeria

Botryosphaeriaceae

Botryosphaeria

Botryosphaeriaceae

gb|AY259098.2|  Botryosphaeria parva 837    0.0 

Heisteria

Daldinia

Xylariaceae

Hypoxylon

Xylariaceae

emb|AJ390409.1|HAN390409  Hypoxylon stygium 1121    0.0  

Heisteria

Daldinia

Xylariaceae

Nodulisporium

Xylariaceae

gb|AY183368.1|  Xylarialean sp. MIRMI 731    0.0 

Ouratea

Fusarium

Nectriaceae

Fusarium

Nectriaceae

gb|AF413049.1|AF413049  Fungal endophyte MS6 915    0.0  

Ouratea

Lepteutypa

Amphisphaeriaceae

Discostroma

Amphisphaeriaceae

gb|AF377285.1|  Discostroma tricellulare 765    0.0 

Heisteria

Phaeoacremonium

Calosphaeriaceae

Phaeoacremonium

Calosphaeriaceae

gb|AF197975.1|AF197975  Phaeoacremonium 400    2e-108

Ouratea

Rosellinia

Xylariaceae

Xylaria

Xylariaceae

gb|AY601897.1|  Fungal endophyte sp. J26 806    0.0 

Heisteria

Scleromitrula

Sclerotiniaceae

Guignardia

Botryosphaeriaceae

gb|AY601899.1|  Fungal endophyte sp. J30 1202    0.0 

LITERATURE CITED

Arnold , A. E., D. A. Henk, R. L. Eells, F. Lutzoni, and R. Vilgalys. 2007. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia, in press.

Higgins, K. L., A. E. Arnold, J. Miadlikowska, S. D. Sarvate, and F. Lutzoni. 2007. Phylogenetic relationships, host affinity, and geographic structure of boreal and arctic endophytes from three major plant lineages. Molecular Phylogenetics and Evolution, in press.



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