Appendix G. Instability in BLAST-based identifications at the genus and family levels for foliar endophytes from two focal tropical hosts (Heisteria concinna, Ouratea lucens).
Primary BLAST analyses were performed in January 2001. The same sequences were subjected to BLAST analysis in August 2005. Changes in genus- and family-level identifications are common, reflecting the growth and refinement of the GenBank database. Recent BLAST results also recover a large proportion of unidentified taxa, reflecting the inclusion of a growing number of environmental samples and unidentified fungi in GenBank. Placement based on 2005 BLAST analyses was confirmed for isolates using phylogenetic methods (Arnold et al. 2007, Higgins et al. 2007).
Host |
Genus (2001) |
Family (2001) |
Genus (2005) |
Family (2005) |
Top match (2005) with match score and e-value |
|
Heisteria |
Arthrinium |
Apiosporaceae |
Pestalotiopsis |
Amphisphaeriaceae |
gb|AF409991.1| Pestalotiopsis sp. 673 0.0 |
|
Heisteria |
Arthrinium |
Apiosporaceae |
Pestalotiopsis |
Amphisphaeriaceae |
gb|AY681481.1| Pestalotiopsis theae 687 0.0 |
|
Ouratea |
Arthrinium |
Apiosporaceae |
Xylaria |
Xylariaceae |
gb|AF153740.1|AF153740 Xylaria sp. MS1033 887 0.0 |
|
Heisteria |
Arthrinium |
Apiosporaceae |
Hypoxylon |
Xylariaceae |
emb|AJ390409.1|HAN390409 Hypoxylon stygium 1096 0.0 |
|
Ouratea |
Botryosphaeria |
Botryosphaeriaceae |
Guignardia |
Botryosphaeriaceae |
gb|AY601899.1| Fungal endophyte sp. 1185 0.0 |
|
Ouratea |
Botryosphaeria |
Botryosphaeriaceae |
Phyllosticta |
Botryosphaeriaceae |
gb|AF532314.1| Phyllosticta sp. MSR-1 850 0.0 |
|
Ouratea |
Botryosphaeria |
Botryosphaeriaceae |
Botryosphaeria |
Botryosphaeriaceae |
gb|AY259098.2| Botryosphaeria parva 837 0.0 |
|
Heisteria |
Daldinia |
Xylariaceae |
Hypoxylon |
Xylariaceae |
emb|AJ390409.1|HAN390409 Hypoxylon stygium 1121 0.0 |
|
Heisteria |
Daldinia |
Xylariaceae |
Nodulisporium |
Xylariaceae |
gb|AY183368.1| Xylarialean sp. MIRMI 731 0.0 |
|
Ouratea |
Fusarium |
Nectriaceae |
Fusarium |
Nectriaceae |
gb|AF413049.1|AF413049 Fungal endophyte MS6 915 0.0 |
|
Ouratea |
Lepteutypa |
Amphisphaeriaceae |
Discostroma |
Amphisphaeriaceae |
gb|AF377285.1| Discostroma tricellulare 765 0.0 |
|
Heisteria |
Phaeoacremonium |
Calosphaeriaceae |
Phaeoacremonium |
Calosphaeriaceae |
gb|AF197975.1|AF197975 Phaeoacremonium 400 2e-108 |
|
Ouratea |
Rosellinia |
Xylariaceae |
Xylaria |
Xylariaceae |
gb|AY601897.1| Fungal endophyte sp. J26 806 0.0 |
|
Heisteria |
Scleromitrula |
Sclerotiniaceae |
Guignardia |
Botryosphaeriaceae |
gb|AY601899.1| Fungal endophyte sp. J30 1202 0.0 |
LITERATURE CITED
Arnold , A. E., D. A. Henk, R. L. Eells, F. Lutzoni, and R. Vilgalys. 2007. Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR. Mycologia, in press.
Higgins, K. L., A. E. Arnold, J. Miadlikowska, S. D. Sarvate, and F. Lutzoni. 2007. Phylogenetic relationships, host affinity, and geographic structure of boreal and arctic endophytes from three major plant lineages. Molecular Phylogenetics and Evolution, in press.