Appendix A. Summary of measurements taken from the mesocosms in the field experiment for each treatment, summaries for the repeated-measures analysis of variance (RM-ANOVA) for each response variable in the field experiment, and summaries for the two-way analysis of variance (ANOVA) for cumulative bacterial productivity (BP) and cumulative bacterial respiration (BR) in the field experiment .
TABLE A1. Summary of measurements taken from the mesocosms in the field experiment for each treatment. Dissolved organic carbon (DOC), bacterial productivity (BP), bacterial growth efficiency (BGE), and dissolved oxygen (O2) were measured daily and are expressed as averages across time and replicates within a treatment. Temperature (Temp) and pH were measured on three occasions and are expressed as averages across time and replicates within a treatment. Measurements of chlorophyll a (Chl a), total nitrogen (TN), and total phosphorus (TP), and crustacean zooplankton abundance (Zoop) were obtained on the last day of the experiment. All data represent the mean ± SEM.
Response variable |
Control |
Low Quality DOC |
High Quality DOC |
||
Press |
Pulse |
Press |
Pulse |
||
DOC |
2.4 ± 0.02 |
3.9 ± 0.32 |
5.2 ± 0.379 |
4.1 ± 0.33 |
5.3 ± 0.39 |
BP |
0.77 ± 0.184 |
1.44 ± 0.282 |
4.02 ± 2.135 |
1.70 ± 0.438 |
2.59 ±1.127 |
BR |
2.15 ± 0.831 |
4.43 ± 0.704 |
5.49 ± 0.734 |
3.69 ± 0.601 |
4.43 ± 0.510 |
BGE |
0.30 ± 0.055 |
0.26 ± 0.040 |
0.29 ± 0.053 |
0.30 ± 0.055 |
0.28 ± 0.048 |
O2 |
6.98 ± 0.031 |
7.12 ± 0.058 |
6.88 ± 0.076 |
6.75 ± 0.094 |
6.83 ± 0.039 |
Chl a (mg/L) |
7.69 ± 0.078 |
19.5 ± 1.68 |
18.9 ± 2.36 |
33.7 ± 1.57 |
28.7 ± 2.86 |
TN |
746 ± 73.7 |
827 ± 39.2 |
968 ± 82.8 |
782 ± 35.1 |
793 ± 53.9 |
TP |
6.3 ± 1.32 |
28.1 ±1.29 |
32.4 ± 3.09 |
38.0 ± 2.45 |
39.5 ± 3.59 |
Zoop |
23.1 ± 3.04 |
20.6 ± 2.54 |
29.4 ± 4.27 |
21.2 ± 3.87 |
32.6 ± 4.28 |
Temp |
20.7 ± 0.35 |
20.7 ± 0.32 |
20.7 ± 0.32 |
20.7 ± 0.32 |
20.7 ± 0.32 |
pH |
7.2 ± 0.07 |
7.0 ± 0.08 |
7.0 ± 0.04 |
6.9 ± 0.08 |
7.2 ± 0.11 |
TABLE A2. Summaries for the repeated-measures analysis of variance (RM-ANOVA) for each response variable in the field experiment.
Response: DOC |
covariance structure: compound symmetry |
|||
Effect |
Num df |
Den df |
F value |
P |
time |
11 |
131 |
1919.17 |
<0.0001 |
quality |
1 |
13 |
6.27 |
0.0263 |
schedule |
1 |
13 |
666.23 |
<0.0001 |
time × quality |
11 |
131 |
1.95 |
0.0386 |
time × schedule |
11 |
131 |
595.9 |
<0.0001 |
time × quality × schedule |
12 |
131 |
2.06 |
0.0239 |
Response: BP |
covariance structure: unstructured |
|||
Effect |
Num df |
Den df |
F value |
P |
time |
11 |
13 |
2519.41 |
<0.0001 |
quality |
1 |
13 |
2 |
0.1812 |
schedule |
1 |
13 |
20.56 |
0.0006 |
time × quality |
11 |
13 |
368.42 |
<0.0001 |
time × schedule |
11 |
13 |
999.9 |
<0.0001 |
time × quality × schedule |
12 |
13 |
6686.35 |
<0.0001 |
Response: BR |
covariance structure: compound symmetry |
|||
Effect |
Num df |
Den df |
F value |
P |
time |
11 |
131 |
5.57 |
<0.0001 |
quality |
1 |
13 |
3.65 |
0.0782 |
schedule |
1 |
13 |
1.99 |
0.182 |
quality × time |
11 |
131 |
0.91 |
0.53 |
schedule × time |
11 |
131 |
2.28 |
0.014 |
quality × schedule × time |
12 |
131 |
1.55 |
0.1159 |
Response: BGE |
covariance structure: unstructured |
|||
Effect |
Num df |
Den df |
F value |
P |
time |
11 |
117 |
6.13 |
<0.0001 |
quality |
1 |
13 |
0.04 |
0.8367 |
schedule |
1 |
13 |
0.02 |
0.883 |
quality × time |
11 |
117 |
0.49 |
0.9065 |
schedule × time |
11 |
117 |
0.32 |
0.9803 |
quality × schedule × time |
12 |
117 |
0.78 |
0.666 |
TABLE A3. Summaries for the two-way analysis of variance (ANOVA) for cumulative bacterial productivity (BP) and cumulative bacterial respiration (BR) in the field experiment.
Cumulative BP, two-way ANOVA |
|||||
Effect |
Num df |
Den df |
SS |
F value |
P |
Quality |
1 |
1 |
178.6 |
2.93 |
0.1127 |
Schedule |
1 |
1 |
1436.2 |
23.56 |
0.0004 |
Quality × Schedule |
1 |
1 |
331.7 |
5.44 |
0.0379 |
Cumulative BR, two-way ANOVA |
|||||
Effect |
Num DF |
Den DF |
SS |
F value |
P |
Quality |
1 |
1 |
282.9 |
2.78 |
0.1212 |
Schedule |
1 |
1 |
440.1 |
4.33 |
0.0596 |
Quality × Schedule |
1 |
1 |
7.7 |
0.08 |
0.7882 |