Ecological Archives E095-049-A1

Sami J. Taipale, Michael T. Brett, Martin W. Hahn, Dominik Martin-Creuzburg, Sean Yeung, Minna Hiltunen, Ursula Strandberg, Paula Kankaala. 2014. Differing Daphnia magna assimilation efficiencies for terrestrial, bacterial, and algal carbon and fatty acids. Ecology 95:564–577. http://dx.doi.org/10.1890/13-0650.1

Appendix A. Detailed concentration information of added nutrients in batch and life table experiments.

Table A1. Experimental setup. The amount of nitrogen (N, µg), phosphorus (P, µg), sterols (mg), essential ω-3 and ω-6 fatty acids (EFA, µg) available in the diets every other day when total carbon content was 5 mg C/L. VicMUa1 = Actinobacterium Candidatus Rhodoluna limnophila (MWH-VicMua1), Mekk-D6 = Polynucleobacter necessarius ssp. Asymbioticus (MWH-Mekk-D6). Particles of Phragmites australis were diluted with either L16 (t-POC L16) medium or lake water (t-POC Lake) and incubated for two months.

Pure or mixed diet

Proportions in diet (%)

N (µg)

P (µg)

Sterols  (µg)

EFA ω-3 (µg)

EFA ω-6 (µg)

Life Table Experiments

 

 

 

 

 

Cryptomonas marssoni

100

710

210

27

266

27

Chlamydomonas sp.

100

830

90

31

191

38

Scenedesmus gracilis

100

630

110

42

342

12

t-POC L16

100

200

60

8

0.03

0.2

t-POC L16/Cryptomonas

98/2

210

63

9

5

1

t-POC L16/Cryptomonas

75/25

328

98

13

67

7

t-POC Lake

100

260

60

6

0.09

0.5

t-POC Lake/Cryptomonas

98/2

269

63

7

5

1

t-POC Lake/Cryptomonas

75/25

373

98

12

67

7

VicMua1

100

1250

270

0

0

0

VicMua1/Cryptomonas

98/2

1239

269

1

4

1

VicMua1/Cryptomonas

75/25

1115

255

7

67

7

Mekk-D6

100

1250

590

0

0

0

Mekk-D6/Cryptomonas

98/2

1239

582

1

5

1

Mekk-D6/Cryptomonas

75/25

1115

495

7

67

7

t-POC L16/VicMua1/Mekk-D6/Cryptomonas

48/20/20/2

1294

956

5

6

1

 

 

 

 

 

 

 

Batch Experiments

 

 

 

 

 

 

Cryptomonas

100

710

0.2

28

385

15

Scenedesmus

100

630

0.1

43

215

62

t-POC L16

100

200

0.1

8

0

0

t-POC L16/Cryptomonas

95/5

226

0.1

9

19

1

t-POC L16/Scenedesmus

95/5

222

0.1

10

11

2.7

t-POC Lake

100

260

0.1

6

0

1

t-POC Lake/Cryptomonas

95/5

283

0.1

7

19

1

t-POC Lake/Scenedesmus

95/5

279

0.1

8

11

4


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